Characterization of theiin vitro/iCytotoxic Assay of Immune Lymphoid Cells in Experimental Allergic Encephalomyelitis (EAE)

摘要

Complement, antilymphocyte globulin, antiencephalitogenic antigen serum, actinomycin D and mitoniycin C inhibited the cytotoxicity against syngeneic fibroblasts of spleen cells more than lymph node cells. The results strongly suggest that the immune cell populations in EAE in the spleen and lymph nodes are qualitatively and/or quantitatively different. Cytotoxicity was dependent upon the number of immune cells in the test. There was evidence of antigenic competition between encephalitogenic antigen and Mycobacterium tuberculosis in the induction of EAE.