Evidence of Contamination in PCR Laboratory Disposables

摘要

Due to its sensitivity, PCR technology is highly susceptible to contamination. Numerous precautions are known and widely taken to reduce contamination risks. The most common type of contamination is product carry over. Other contamination stems from isolated DNA or cellular material. While product carry over is of the laboratory's own making, this is not necessarily true for contamination either with isolated DNA or cellular material. Regardless its type, contaminating DNA may enter the PCR process through different routes: within the sample itself, in chemical reagents, on laboratory disposables, or through intake from air at any stage of sample processing.