Cell-free extracts have been prepared from virtually pure cultures ofEpidinium ecaudatumCrawley, a protozoon which may be abundant in the rumen of cows feeding on red clover. Water extracts contain an amylase while phosphate buffer extracts contain the same amylase together with a less soluble maltase and occasionally a cellobiase. Invertase and #x3B2;-glucosidase were absent from the extracts which were also unable to hydrolyse lactose, melibiose, #x3B1;-trehalose and cellulose.
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