Homogeneous Enzyme Immunoassay lpar;EMITrpar; Protocol for Monitoring Tricyclic Antidepressants on the COBAShyphen;BIO Centrifugal Analyzer

摘要

EMITreg; tests are available for quantitative determination of the tricyclic antidepressants amitriptyline (AMI), nortriptyline (NORT), imipramine (IMI), and desipramine (DMI). An extraction step before analysis eliminates cross-reacting polar metabolites. Excellent correlation between EMIT and high-performance liquid chromatography (HPLC) has been previously established. Most published protocols for using EMIT reagents on COBAS-BIO centrifugal analyzers were designed for analytes present at 1ndash;50 mg/L in serum. AMI, NORT, IMI, and DMI are usually present at far less than 1 mg/L. We describe a COBAS-BIO EMIT protocol for assaying these analytes. Patient sample correlation between COBAS-BIO EMIT and EMIT-AutoLab were 0.99. Between-run precision for single-point determinations was comparable to SYVA AutoLab performance le; 11percnt; at 40 mu;g/L AMI, NORT, IMI (80 mu;g/L DMI), and le;4percnt; at 200 mu;g/L AMI, NORT, IMI (400 mu;g/L DMI). With stored-curve updating, working reagents were usable for at least 14 days (AMI) or 23 days (DMI).