Contributions of separation and dispersion to the total zone width of a polydisperse latex sample under various flow rates and retentions were studied. Both the direct flow and the reverse flow techniques were employed and the experimental results were compared with theoretical relationships. The results showed a good agreement between the theory and the experiment under different conditions. A good agreement between the mean particle diameters calculated from our experimental data and those given by the producer was found. The width of the particle size distribution characterized by the standard deviation was found about two fold higher than that given by the producer.
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