Mathematical Modeling of Various Amines in the Ion-paired Liquid Chromatographic Analysis of Bilirubin

摘要

The use of amines as ion-paired agents for bilirubin is explored. The effect of amine type, amine concentration, hydrogen ion concentration, and methanol concentration are determined and modeled by equations. The ability to adjust the capacity factor using multiple variations of the mobile phase should be useful in optimizing the separation of bilirubin from other components in various biological and theoretical studies of this interesting bile pigment.