The cytostatic effects of conventional high osmolal ionic contrast media (meglumine-calcium metrizoate and Na-metrizoate) and new low osmolal nonionic contrast media (iohexol and iopamidol) in synchronized cell cultures were tested. The cell-cycle prolongation was most pronounced when the contrast media were added in the G1phase, but there was also a marked effect when the contrast media were added in the S phase or late in the G2phase. The cytostatic effect even persisted into the first cell cycle following the termination of the exposure.All four contrast media exerted effects stronger than that of equiosmolal saline. Iohexol and iopamidol produced a more severe effect than meglumine-calcium metrizoate and Na-metrizoate at equal osmolality. Thus, the cytostatic effect of contrast media cannot be explained only by hypertonicity; the contrast media must have an additional specific cytostatic effect.When the cytostatic effect was related to iodine concentration, the new low osmolal nonionic contrast media influenced the cell cycle less than the conventional high osmolal ionic contrast media.
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机译:测试了常规高渗透压离子造影剂(葡甲胺-甲硫酸钙和甲硝酸钠)和新型低渗透压非离子造影剂(碘海醇和碘帕醇)在同步细胞培养物中的细胞抑制作用。在 G1 期添加造影剂时,细胞周期延长最为明显,但在 S 期或 G2 期后期添加造影剂时也有显着效果。细胞抑制作用甚至持续到暴露终止后的第一个细胞周期。所有四种造影剂的效果都比等渗盐水强。碘己醇和碘帕米醇在同等渗透压下比葡甲胺-甲磺酸钙和甲硝酸钠产生更严重的作用。因此,造影剂的细胞抑制作用不能仅用高渗性来解释;造影剂必须具有额外的特异性细胞抑制作用。当细胞抑制作用与碘浓度有关时,新型低渗透压非离子造影剂对细胞周期的影响小于常规高渗透压离子造影剂。
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