Genomic clones corresponding to class II β genes of theSLAchaplotype of miniature swine have been isolated and characterized. These genes have been grouped into seven non-overlapping clusters on the basis of restriction mapping. Ordering of exons within each cluster was accomplished by hybridization of Southern blots of restriction fragments with exon-specific probes. The two clusters (clusters 2 and 3) encoding theDRBandDQBgenes were identified on the basis of hybridization with locus-specific 3′ untranslated cDNA probes. Cluster 4 contained exons of bothDOBandDQBgenes, the basis for which remains to be determined. The remaining four clusters (1, 5, 6, 7) were identified as containingDP, DR, andDOcoding sequences, respectively, on the basis of sequence analysis. The porcine class II region appears very similar to that of man in number and nature of the class II genes identified and in the intron/exon organization of corresponding gen
展开▼
