Possible mutagenic effects of low frequency (20 kHz) ultrasound have been assessed with genetic systems ofBacillus subtilis. Ultrasound was unable to cause a detectable increase in the spontaneous frequency of back‐mutation irrespective of the degree of killing. Similar treatments were incapable of producing mutagenic lesions that could be detected by the system of transformation afterin vitrotreatment of DNA. Transforming activity and molecular weight could be reduced without a corresponding decline in linkage between two contiguous markers. It is concluded that mutagenic effects of ultrasound could not be detected using these genetic system
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