A careful forensic examination of the sexually abused child may reveal evidence of male ejaculation if this had occurred in the recent past. Two cases of the unexpected presence of vaginal sperm in prepubertal children are presented. The techniques of sperm and acid phosphatase collection are discussed. Sperm and/or other seminal products should be sought in victims of recent assault, in preverbal or nonverbal children, in victims with signs or symptoms of recent penetration, or in any case where the recency of sexual contact is unknown or uncertain.
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