Green Synthesis of Zinc Oxide Nanoparticles Mediated from Cassia renigera Bark and Detect Its Effects on Four Varieties of Rice

摘要

The present study reports a cost-effective and environmentally benign method for biogenic synthesis of ZnO nanoparticles (NPs) using Cassia renigera bark extract (CRBE) and evaluation of its effect on four rice varieties (PR 7, basmati 1509, HR 47, and sharbati). The physicochemical characterisation of CRBE: ZnO NPs was carried out using X-ray diffractometer (XRD), Field emission scanning electron microscope (FESEM) and UV-Vis spectrophotometer. Structural investigation using Rietveld refinement method revealed hexagonal wurtzite structure of CRBE: ZnO NPs containing P6 3 mc space group. UV- Visible spectra of CRBE: ZnO NPs displayed a broad peak ranging from 430-540 nm. Fourier transform infrared (FTIR) spectra of CRBE: ZnO NPs displayed a broad peak at 3325 cm(-1) which represent amine group and the bend at 432 cm(-1) showed Zn-O stretching confirming the formation of ZnO NPs. FESEM micrograph of CRBE: ZnO NPs depicted hexagonal grains of varying size distributions with an average grain size of similar to 28 nm. The effect of CRBE: ZnO NPs on the growth of different rice varieties was examined and was compared with the untreated seeds of rice. CRBE: ZnO NPs treated seeds of rice varieties displayed a significant increase in germination rate, moisture rate, and growth rate (shoot/root length, number of leaves) w.r.t. untreated seeds. CRBE: ZnO NPs mediated rice seeds possessed anti-pathogenic activity against Xanthomonas oryzae (Xoo). The results of the present work represent that CRBE: ZnO NPs can serve as potent nano-fertilizer in the field of agriculture.