Nitric oxide participates in the regulation of ascorbate-glutathione cycle and water physiological characteristics of Arabidopsis thaliana by NaHS

摘要

We investigated the role of nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle and water physiological characteristics of Arabidopsis thaliana by sodium hydrosulfide (NaHS). NaHS markedly increased the contents of H 2 S, NO, chlorophyll (Chl), and carotenoids, the activity of AsA-GSH cycle, ascorbate/dehydroascorbate ratio, net photosynthetic rate, Chl fluorescence parameters, transpiration rate, stomatal conductance, and relative water content in leaves and the biomass of wild-type Arabidopsis . However, NaHS markedly decreased malondialdehyde content and electrolytic leakage. Except H 2 S, above NaHS-induced promotions were suppressed by nitrate reductase (NR) inhibitor sodium azide (NaN 3 ). Application of sodium nitroprusside (SNP) to (NaN 3 NaHS)-treated wild type Arabidopsis (NaN 3 SNP NaHS) reversed above effects of NaN 3 NaHS. However, NaN 3 NaHS and NaN 3 SNP NaHS had no significant effects on H 2 S content. Meanwhile, we proved above results by using NO-associated NR gene mutant nia1,nia2 . Above results suggested that NO participated in the regulation of AsA-GSH cycle and water physiological characteristics of Arabidopsis by NaHS.