In this present study, a total of 43 clinical samples were collected from the desi birds of the southern Tamil Nadu region showing swelling in the infraorbital sinus and suspected for coryza infection. Samples were inoculated into the Chocolate agar and kept at 37℃ for 48 hrs, of which 05 isolates showed the typical dewdrop colonies in Chocolate agar culture, were further confirmed by the biochemical test and HPG-2 PCR. Direct HPG-2 PCR was also performed in the 43 clinical samples, of which 17 samples were identified as Avibacterium paragallinarum by producing a specific amplicon of 500bp. HPG-2 PCR positive samples were further subjected for serotype identification by serotype-specific PCR targeting the HMTp210 region and 03 samples were identified as serovar A by producing an amplicon of 0.8 kbp, and 14 samples were identified as serovar B by producing an amplicon of 1.1 kbp. But, none of the samples amplified the product for serovar C. This study concludes that the more prevalent circulating serovar in the southern Tamil Nadu region is serovar B, followed by serovar A. To the best of the author's knowledge, this is the second report stating the presence of serovar B in India.
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