New insight on the mechanism of eating disorder in females based on metabolic differences of bisphenol S in female and male zebrafish

摘要

The different capacity of glucuronic acid metabolism might lead to the difference of bisphenol S (BPS) residual in tissues of male and female zebrafish. This may be the fundamental reason why BPS causes different effects in females and males. Here, adult zebrafish are exposed to 1, 10 and 100 mu g/L BPS for 35 days to explore the main effect on females. After exposure, the liver of females showed stronger BPS metabolism ability than males, resulting in the accumulation of BPS in the gut of females. The results of neurotransmitters in gut of females revealed that the content of serotonin was decreased by BPS treatments. In addition, the mRNA expression levels of tryptophan 5-monooxygenase (Tph1) that regulated serotonin synthesis was reduced in gut of females in all BPS groups, and Tph1 protein has very high affinity with BPS molecule. Adult females treated with BPS exhibited symptoms including overeating, a decrease of serotonin in the gut, hypoglycemia and hyperlipidemia, a similar effect of Tph1 protein inhibitor LP533401 on adult females. This hypoglycemia stimulates brain agrp/pomc and orexin neurons to induce overfeeding behavior, causing intestinal homeostasis imbalance and hyperlipidemia. Our data elucidate a potential pathogenesis of eating disorder under pollutant stress.