Abstract Aims In the context of minor orthodontic intervention using clear aligner technologies, we determined antimicrobial properties of a cellulose‐based material loaded with essential oils such as cinnamaldehyde. Methods and Results Isothermal microcalorimetry was used to assess the growth of bacterial biofilms at the interface between the tested material and the solid growth medium. The calorimetric data were analyzed using conventional growth models (Gompertz and Richards), and inhibition at 12 and 24 h was calculated. Conclusions The tested material showed antimicrobial properties against Staphylococcus epidermidis as well as Streptococcus mutans and Streptococcus mitis clinical isolates. The inhibition was more pronounced against S. epidermidis, for which growth rate was reduced by 70 and lag phase was extended by 12 h. For S. mutans and S. mitis, the decrease in growth rate was 20 and 10, and the lag phase increased by 2 and 6 h, respectively. Significance and Impact Clear aligners for minor teeth alignment are becoming very popular. As they must be worn for at least 22 h per day for up to 40 weeks, it is important that they remain clean and do not promote caries formation or other oral infections. Therefore, introducing material with antimicrobial properties is expected to maintain oral hygiene during the aligner therapy. Here, we demonstrate the use of cinnamaldehyde for reducing microbial growth and biofilm formation on cellulose‐based dental clear aligners.
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