Aphids are considered as most economically importance and worldwide insect pests. Successful pest management systems are based on accurate and rapid pests' species identification. Traditional morphological identification of closed aphid species may be considered as inaccurate taxonomic process. For overcoming disadvantages of traditional morphological identification, molecular techniques, related to DNA markers and based on polymerase chain reaction (PCR), were approached by using nine ISSRs primers to identify and diagnose fifteen common aphid species that disperse in Egyptian agro-ecosystem. The examined ISSRs primers could successfully discriminate the tested aphid species that reflected 61.39 polymorphism among them. Moreover, four banding patterns were considered as unique bands which could characterize three aphid species (Aphis gossypii, Aphis nerii and Myzus persicae). Highest genetic homology (84.9) was observed between species Rhopalosipum padi and Schizaphis graminum. In additions, each of A. gossypii and Aphis citricola were also genetically homologous species. In contrast, species Aphis craccivora and M. persicae were analogous genetically with low similarity percentile (59.8). High genetic divergence was observed also between A. nerii and M. persicae. Two alternative molecular branching taxonomic keys were proposed by subjecting five highest polymorphic ISSRs primers and 29 banding patterns with different molecular sizes.
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机译：蚜虫被认为是最具经济意义和世界范围的害虫。成功的害虫管理系统基于准确和快速的害虫物种识别。封闭蚜虫物种的传统形态鉴定可能被认为是不准确的分类过程。为了克服传统形态学鉴定的缺点，采用与DNA标记相关的分子技术，基于聚合酶链反应（PCR），使用9种ISSRs引物鉴定和诊断15种分布在埃及农业生态系统中的常见蚜虫物种。所研究的ISSRs引物能够成功区分被试蚜虫物种，其中蚜虫的多态性为61.39%。此外，4种条带模式被认为是独特的条带，可以表征3种蚜虫（棉蚜、棉蚜和蚜虫）。Rhopalosipum padi和Schizaphis graminum物种之间的遗传同源性最高（84.9%）。此外，棉蚜（A. gossypii）和蚜蚜（Aphis citricola）在遗传上也是同源种。相比之下，Aphis craccivora 和 M. persicae 物种在遗传上相似，相似性百分位数较低 （59.8%）。在 A. nerii 和 M. persicae 之间也观察到高度的遗传差异。通过对5个最高多态性ISSRs引物和29个不同分子大小的条带模式进行研究，提出了两种替代的分子分支分类键。
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