Colletotrichum okinawense, the causal agent of postharvest anthracnose fruit rot of papaya in Taiwan

摘要

Anthracnose fruit rot is the main postharvest disease ofpapaya (Carica papaya) in Taiwan. Locally produced papaya fruits were collected in markets in Chiayi (23°28′44.1”N, 120°27′26.7″E), Taiwan in 2018 and stored at25–28 °C. After 5 days, symptoms of sunken watersoaked necrotic lesions partly covered with white mycelium developed, similar to the description of Duan et al.(2018). A fungus was isolated from symptomatic fruit,and two representative isolates, PP1 and PP3, were obtained. The isolates formed white colonies with salmoncolored conidial masses on PDA. The conidia wereaseptate, hyaline, cylindrical, and measured 13.6 ± 1.6(8.3–17.1) × 5.2 ± 0.5 (4.2–6.4) μm. The isolates were further identified by sequencing the internal transcribedspacer (ITS) region of the rDNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), andbeta-tubulin (TUB2) genes (Damm et al. 2019). The sequences were deposited in GenBank under accession Nos.MK649934–MK649935 and MK790068–MK790073. TheITS, GAPDH, ACT, and TUB2 sequences shared 100identity with those of Colletotrichum okinawense extype strain MAFF 240517T (MG600767, MG600827,MG600971 and MG601034) by BLASTn searches.