Detection of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) DNA in endocervical samples from a positive and negative HPV woman of Cordoba, Argentina

摘要

Aims The purpose of the present study was to elucidate the presence of human herpesvirus 6A (HHV-6A), HHV-6B and HHV-7 in samples of the uterine cervix through detection of viral DNA. We analysed normal tissues, samples with low-grade squamous intraepithelial lesions (LSILs) and high-grade squamous intraepithelial lesions (HSILs). We correlated the presence of HHV-6 and HHV-7 with the finding of human papillomavirus (HPV) in mucosal samples. Methods Cervical samples were examined and grouped as follows: group 1 (n=29), normal cytology; group 2 (n=61), samples with LSIL; group 3 (n=35), samples with HSIL. Molecular biology examinations were performed in all samples to detect HHV-6, HHV-7 and HPV DNA and to typify HHV-6 species. Results Group 1: normal cytology and HPV (-): HHV-6: 6.8 (2/29), HHV-7: 79.3 (23/29); group 2: LSIL and HPV (-): HHV-6: 93.1 (27/29), HHV-7: 96.5 (28/29); LSIL and HPV (+): HHV-6: 0 (0/32), HHV-7: 90.6 (29/32); group 3: HSIL and HPV (-): HHV-6: 20 (2/10), HHV-7: 70 (7/10); HSIL HPV (+): HHV-6: 12 (3/25), HHV-7: 68 (17/25). HHV-6A DNA was not detected in any samples. Conclusions (1) Both HHV-6 and HHV-7 infect the mucosal cells of the cervix with higher prevalence of HHV-7. (2) The higher prevalence of HHV-6 in LSIL HPV (-) samples compared with those with normal cytology indicates that it constitutes a possible risk factor for atypia production. (3) The presence of HHV-7 in all samples questions its role in the production of atypia. (4) The finding of HHV-6 and HHV-7 suggests that the cervical mucosa is a possible transmission pathway for these viruses.