FPX-113 attenuates inflammatory responses by deteriorating cytokines, neutrophil activity and mast cell degranulation via Akt/ NF- κB pathway

摘要

Search of novel chemical compounds to manage disorders of inflammation is always on demand. Herein we report FPX-113 a novel small molecule to exhibit excellent anti-inflammatory properties in human whole blood (HWB), peripheral blood mononuclear cells (PBMCs), RBL-2H3 and THP-1 cells. FPX-113 showed an inhibition of TNF-α, IFN-γ, IL-2, IL-6, IL-8 and IL-17 release fromHWB and PBMC with IC_(50) values of 364.6 nM, 505.2 nM, 160.2 nM, 369.1 nM 371.2 nM, 459 nM and 64.14 nM, 92.30 nM, 49.88 nM, 151.7 nM, 108.9 nM, 92.70 nM respectively. The compound inhibited neutrophil migration and elastase in a dose dependent way. Further, FPX-113 inhibited the degranulation of rat mast cell basophils in RBL-2H3 cells with an IC_(50) of 280.5 nM. A dose dependent inhibition of Akt phosphorylation in RBL-2H3 and THP-1 cells and down regulation of NF- κB in PBMCs, RBL-2H3 and THP-1 cells by FPX-113 were evidenced when induced with 5 μg/mL PHA + 50 ng/mL PMA for 30 min. Taken together, the results suggest FPX-113 exhibits anti-inflammatory mechanism signaled via Akt/ NF- κB pathway. These findings will provide a wide scope for FPX-113 and its close analogues to be developed as novel anti-inflammatory agents for various diseases.