Prevalence of Waterborne blaNDM-1 Gene Producing Carbapenem-resistant Klebsiella pneumoniae from Al-Hillah River Water, Babylon Province, iraq

摘要

© The Author(s) 2022.The current work suggested the occurrence of blaNDM-1 gene among Klebsiella pneumoniae recovered from surface waters of the Al-Hillah River. Between January and April 2015, water samples (101) were taken from seven different area of the Al-Hillah River, Babylon province, iraq. K.pneumoniae was reported in percentage of 35 (34.6). the antibiotics susceptibility profile of K.pneumoniae was determined with disk diffusion assay. the most common resistance was detected for penicillins agents (ampicillin and cloxacillin) with 20(57.14) and 17(48.57) resistance rate, respectively. two isolates of K.pneumoniae were carbapenem-resistant. Phenotypic screening of metallo β-lactamase detection was carried out using imipenem–eDtA double disk synergy test for carbapenem resistant isolates, 2(100) isolates with positive result. Conventional Polymerase Chain Reaction (PCR) test was used for detection NDM-1 beta-lactamase, 1 (50) K.pneumoniae isolate harboring this gene.