High-Performance Liquid Chromatographic Determination of Lanatosides inDigitalis LuteaandDigitalis AmbiguaLeaves

摘要

An quantitative method for the determination of lanatoside A and lanatoside B inDigitalis luteaandDigitalis ambigualeaves by high-performance liquid chromatography (HPLC) is described. The extract of dry leaf powder with chloroform:ethanol (1:2, v/v) was submitted to Sep-Pak cartridges prior to HPLC analysis. HPLC was performed on an ODS column using methanol:water (2:1, v/v) forDigitalis luteaand a phenylsilyl bonded silica column with acetonitrile:water (5:8, v/v) forDigitalis ambigua. The effluent was monitored by ultraviolet (UV) absorption at 220 nm. The quantitation was carried out by the internal standard method. The present method is sufficiently sensitive and reproducible to assay lanatosides inDigitalisleaves.