Phlorotannin-coated poly (epsilon-caprolactone) film as a potential material for postsurgical adhesion prevention

摘要

Phlorotannins are known to possess several therapeutic properties such as anti-inflammatory and anti-oxidant effects, both of which are related to prevent tissue adhesion. Therefore, this study sought to fabricate phlorotannin-coated PCL films for the prevention of post-surgical adhesion. Phlorotannin-coated PCL films were fabricated via the solvent casting and coating methods. After which the fabricated film was characterized using water contact angle analysis, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and drug release ability analysis. Furthermore, the anti-inflammatory and cell proliferation-inhibiting properties of the fabricated film were assessed in LPS-stimulated RAW264.7 macrophage cells and NHDF-neo cells using the nitric oxide production assay, the WST-1 assay kit, and Hoechst 33342 dye. The results indicated that the phlorotannin-coated PCL film significantly inhibited nitric oxide production and reduced NHDF-neo cell proliferation. These results suggest that phlorotannin-coated PCL films constitute a promising anti-adhesive bioactive compound with applicability in the field of DDSs.