Abstract β‐galactosidase (β‐gal) is one of the most prevalent markers of gene expression. Its activity can be monitored via optical and fluorescence microscopy, electrochemistry, and many other ways after slight modification using protein engineering. Here, we have constructed a chimeric version that incorporates a streptococcal protein G domain at the N‐terminus of β‐gal that binds immunoglobins, namely IgG. This protein G: β‐galactosidase fusion enables β‐gal‐based spectrophotometric and electrochemical measurements of IgG. Moreover, our results show linearity over an industrially relevant range. We demonstrate applicability with rapid spectroelectrochemical detection of IgG in several formats including using an electrochemical sensing interface that is rapidly assembled directly onto electrodes for incorporation into biohybrid devices. The fusion protein enables sensitive, linear, and rapid responses, and in our case, makes IgG measurements quite robust and simple, expanding the molecular diagnostics toolkit for biological measurement.
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机译：摘要 β-半乳糖苷酶（β-gal）是最普遍的基因表达标志物之一。它的活性可以通过光学和荧光显微镜、电化学和许多其他方式进行监测，使用蛋白质工程进行轻微修饰。在这里，我们构建了一个嵌合版本，该嵌合版本在结合免疫球蛋白（即 IgG）的 β-gal 的 N 末端包含一个链球菌蛋白 G 结构域。这种蛋白质 G：β-半乳糖苷酶融合能够对 IgG 进行基于 β-gal 的分光光度法和电化学测量。此外，我们的结果显示了工业相关范围内的线性。我们证明了对多种形式的 IgG 进行快速光谱电化学检测的适用性，包括使用电化学传感接口，该接口可快速直接组装到电极上以掺入生物混合器件中。融合蛋白可实现灵敏、线性和快速的响应，在我们的案例中，使 IgG 测量非常稳健和简单，扩展了用于生物测量的分子诊断工具包。
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