METHYLATION OF THE E-CADHERIN (ECAD) GENE IN CLEAR CELL RENAL CELL CARCINOMA (CCRCC)

摘要

Clear cell renal carcinoma is one of the most common sub-types of renal cell cancer. Methylation is the leading type of epigenetic changes. Many studies in the field emphasized that hypermethyla- tion of some genes is efficient on the formation of many cancer types. In this study, the aim is to analyze DNA methylation, one of the epigenetic changes in the ECAD gene of renal cell carcinoma (RCC) patients, and to determine the relations by comparing the present epigenetic features with clinic-pathologic parameters. Studies were organized in two groups: a control group with 153 individuals and a patient group with 113 individuals. The methylation of blood samples was determined by displaying in the gel after methylation specific PCR (MSP) steps. Some descriptive statistics and Wilcoxon, Correlation and Logistic Regression analyses were performed using SPSS. As a result of this study, it was found out that the probability of the occurrence of this disease was 5 times higher in people who have methylation in their ECAD genes than those who have not. Furthermore, it was observed that women are 4.253 times more likely to have disease compared to men. This research is a very important and fundamental study on the diagnosis and treatment of CCRCC disease in terms of the effect of epigenetic mechanisms such as methylation analysis.