Screening and Identification of Newly Isolated Basic Red 9-Degrading Bacteria from Textile Wastewater and Their Ability to Produce Medium-Co-Long-Chain-Length Polyhydroxyalkanoates

摘要

Abstract Newly isolated Basic Red 9-degrading bacteria were isolated from textile wastewater using a pretreatment method. Nine strains were isolated; however, only five strains accumulated polyhydroxyalkanoates (PHAs). Thereafter, PHA-producing strains were identified through 16S rDNA sequencing analysis and phylogenetic evaluation and were found to belong to Enterobacter with 100 identification. The five isolated strains were incubated with a PHAs-producing medium containing 100?mg/l Basic Red 9 (BR9) to study decolorization efficiency, and PHAs production. Enterobacter sp. strains TS3 and TS1L effectively decolorized the BR9 dye with degradation rates of 63.43 and 79.15, respectively. PHAs production from TS3 and TS1L was also observed to be 75.34 and 72.32 of dry cell weight (DCW), respectively. Furthermore, Enterobacter sp. strains TS3 and TS1L accumulated medium-co-long-chain-length PHAs (mcl-co-lcl PHAs). This is the first report using Enterobacter strains to degrade BR9 dyes from textile wastewater and to assess their ability to produce mcl-co-lcl PHAs.