In recent years, diclofenac, a non-steroidal anti-inflammatory drug has been found to be causative for adverse reactions in aquatic fauna and hence it has been chosen for analysing its toxic effect. Being an aromatic herb, Artemisia pallens has flavonoids and phenolic compounds that have been employed to check its competency in lessening toxic effects. The fish, Pangasius Sp., were treated with lethal concentration (12 mg/mL for 4 days) of diclofenac to induce liver damage and 1 of Artemisia pallens extract was added. The vacuolar degeneration in the liver was visually observed using histopathological analysis. The oxidative stress and antioxidant parameters were studied. Noteworthy changes in levels of conjugated dienes, protein sulfhydryl, inorganic phosphorus, superoxide dismutase and catalase activities with their assayed results at p<0.01 and p<0.05 level of significance when compared to control fish were observed. The stress hormone, cortisol level reduction proved the fact that the action of Artemisia pallens has lessened the toxic effects of diclofenac leading to a considerable increase in the lifespan of the fish. The observed changes in the oxidative stress parameters, antioxidant enzymes and cortisol levels further confirmed the hepato-protective effect of Artemisia pallens against diclofenac induced toxicity.
展开▼
