In vitro evaluation of cytotoxicity, anti-inflammatory and wound healing properties of otolith stones from pomadasys maculates

摘要

Pomadasys maculatus (Blotched-grunt, saddle grunter) generally, often known as the "Dhoter," is a commercially significant fish of the coast of India-Pakistan. Fishes are high in nutrients such as polyunsaturated fatty acids, amino acids, vitamins, and minerals. It is important in the prevention and treatment of coronary heart disease, asthma, eye problems and vitamin shortages. The natural crystalline material collected from the head of the Pomadasys maculatus fishes are used for various characterization techniques such as single crystal XRD, powder XRD, EDAX, SEM, FT-IR and FT-Raman, and the other biological properties of the Otolith stones are analyzed. The unit cell characteristics of the naturally collected otolith stones were determined using single crystal X-ray diffraction. The FT-IR and FT-Raman spectra investigated the various functional groups found in the otolith stones. The current study also sought to investigate the cytotoxicity, anti-inflammatory and wound healing properties of otolith stones found in Pomadasys maculatus fishes. One of the goals of this study is to determine whether otolith stones have cytotoxic effects on fibroblast cells. The inhibitory effects of otolith stones in LPS-stimulated RAW 264.7 macrophages were used to assess their anti-inflammatory properties. The wound scratch assay assured the otolith stone's wound healing efficacy. The EDAX analysis exhibits the various constituents present in otolith stones.