Remotely Actuated Magnetic Nanocarpets for Bone Tissue Engineering: Non-Invasive Modulation of Mechanosensitive Ion Channels for Enhanced Osteogenesis

摘要

Non-invasive approaches using remotely controllable nanomaterials havedemonstrated their potential ability to enhance treatment efficacy in regenerativemedicine and tissue repair. Although magnetic nanoparticles (MNPs)have been used for multiple healthcare applications where their remotecontrol properties can show significant advances, enhanced surface functionalgroups, and electrical properties would expand their capabilities. Toaddress this, in this study, MNPs incorporated Graphene Oxide (GO) basednanocomposites (GOMNPs) are developed and functionalized with TREK1and Piezo1 antibodies to specifically target the respective mechanosensitiveion channels. Magnetic ion channel activation (MICA) technology is used toremotely activate MG63 osteoblast-like cells tagged with these functionalizedGOMNPs. Remote activation of mechanotransduction pathways showssignificant upregulation in osteogenic gene expression as well as enhancedalkaline phosphate activity and calcium mineralization with enhanced boneformation. The development of a GOMNP composite has extensive applicabilityfor future clinical translation.