Cytotoxicity of black cumin (Nigella sativa L.) seed sprout extract mediated titanium oxide (TiO2) nanoparticles in human lung cancer cells

摘要

We report the preparation and cytotoxicity of TiO2 nanoparticles (TiO2 NPs) using black cumin sprout extract. The average size of BC-TiO2 NP was 97.48 +/- 1.54 d. nm, with a PDI of 0.31, and zeta potential of-29.1 +/- 0.89 mV. The size of NPs varied between the methods (TEM, XRD, and DLS) due to the hydrodynamic properties of BC-TiO2 NPs. The cytotoxicity studies evidenced that BC-TiO2 NPs exhibited a half maximum inhibitory con-centration (IC50) of 76.25 +/- 1.57 mu g/mL for the human lung cancer cell line (A549) and 175.64 +/- 3.48 mu g/mL for the mouse fibroblast cell line (NIH3T3). In addition, BC-TiO2 NPs triggered the antiproliferative activity in the A549 cell line through nucleus damage, mitochondrial membrane potential loss, and apoptosis-associated membrane changes while showing no toxic effects on egg CAM and RBC. These results revealed that BC-TiO2 NPs exhibited strong cytotoxicity in the A549 cell line while being nontoxic to the NIH3T3 cell line, egg CAM, and RBC.