...
首页> 外文期刊>Aquaculture Research >Induced quiescence in eggs of the tropical calanoid copepod Acartia tropica: Effect of different storage conditions
【24h】

Induced quiescence in eggs of the tropical calanoid copepod Acartia tropica: Effect of different storage conditions

机译:热带蠟类桡足类桡足类蛾卵的诱导静止:不同贮藏条件的影响

获取原文
获取原文并翻译 | 示例

摘要

Abstract Copepods of genus Acartia are considered as efficient live feeds for marine fish larviculture. A few reports are already there on production and storage for eggs of few temperate species of Acartia. Present study evaluated the storage potential of eggs of a tropical calanoid copepod, A. tropica. Different conditions examined were higher salinity storage (40–200 ppt) at room temperature, cold storage (4ºC) at different salinities (15–200 ppt), cryoprotectant solutions (methanol, glycerol) and antibiotic solutions (kanamycin, oxytetracycline). Results showed that higher salinity storage (50–200 ppt) could not retain egg viability under cold storage, as well as normal temperature conditions. The eggs exposed to cold storage and lower salinities (15–40 ppt) for 24 h survived with highest egg‐hatching success (EHS, ) recorded at 15 ppt (69.44 ± 8.12). The condition was not efficient to retain egg viability beyond 24 h. The addition of certain cryoprotectants and antibiotics to the storage media significantly improved the survival of A. tropica eggs after 48 h storage. In the cryoprotectant treatments highest EHS after 48 h storage was recorded at 1M Glycerol (41.27 ± 6.34). Amongst the antibiotic treatments highest EHS after 48 and 72 h of storage were recorded at 100 ppm Kanamycin (46.79 ± 10.33, 2.67 ± 0.35) and 50ppm Kanamycin (43.86 ± 12.81, 1.73 ± 0.27), respectively. A drastic reduction in egg viability was recorded after 48 h and the antibiotics and cryoprotectants examined were not efficient to retain egg viability beyond 72 h. Hence the conditions examined in the present study could retain the viability of A. tropica eggs maximum up to 72 h and were not sufficient to induce long term quiescence in A. tropica eggs.
机译:摘要 桡足类动物被认为是海水鱼类幼体养殖的高效活饲料。已经有一些关于少数温带物种的Acartia卵的生产和储存的报告。本研究评估了热带卡兰科桡足类(A. tropica)卵的储存潜力。检查的不同条件是室温下较高盐度储存(40-200 ppt)、不同盐度(15-200 ppt)的冷藏(4ºC)、冷冻保护剂溶液(甲醇、甘油)和抗生素溶液(卡那霉素、土霉素)。结果表明,较高盐度的贮藏(50-200 ppt)不能在冷藏和常温条件下保持鸡蛋的活力。暴露在冷藏和低盐度(15-40 ppt)下24 h的种蛋存活下来,种蛋孵化成功率(EHS,%)最高,为15 ppt(69.44±8.12)。该条件在24小时以上保持鸡蛋活力无效。在贮藏培养基中添加某些冷冻保护剂和抗生素可显著提高热带蛤蜴贮藏48 h后的存活率。在冷冻保护剂处理中,储存 48 小时后的最高 EHS 记录为 1M 甘油 (41.27 ± 6.34)。在抗生素治疗中,储存48小时和72小时后EHS最高的分别为100 ppm卡那霉素(46.79 ± 10.33、2.67 ± 0.35)和50 ppm卡那霉素(43.86 ± 12.81、1.73 ± 0.27)。48 小时后记录到卵子活力急剧下降,并且检查的抗生素和冷冻保护剂不能有效地将卵子活力保持在 72 小时以上。因此,本研究所考察的条件可以保持热带曲霉卵的活力长达72 h,并且不足以诱导热带曲霉卵的长期静止。

著录项

获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号