A Synergic Fabrication of Chitosan-Coated Salinomycin-Loaded Hydroxyapatite Potential Nanocarriers for the Treatment of Liver Cancer

摘要

There are numerous possible implications of nanotechnology in cancer therapy. To improve the therapeutic benefits of anticancer drugs nano-delivery systems (NDS) have been established with significant solubility, degradability and cytocompatibility. These NDS are the result of several advantages that high-performance nanoparticles hydroxyapatite (HA) advance rapidly in specific cancer treatments. Salinomycin is a pharmaceutical drug that serves to suppress tumour cells and the growth of many cancers as a chemical inhibitor. Nevertheless, Salinomycin's low bioavailability is the main challenge to be used. In this work, HA chitosan-coated nanoparticles were utilised to enhance the efficiency and availability of Salinomycin as a pH-sensitive biopolymer. HA nanoparticles were initially produced using the sol-gel technique for this purpose. Then a coating of chitosan was coated with the nanocarrier under regulated circumstances, and the salinomycin medicine was encapsulated. For the characterization of nanocarriers were employed techniques such as scanning electron microscopy, X-ray diffraction, and Fourier transformations infrared spectroscopy. Different bioassays have tested nano-drugs developed in the second part. In this connection, we examined the cytotoxicity rate of the hepatocellular carcinoma HepG2 and Caco-2 by the MTT assay and the apoptosis induction rate by ethidium bromide and acridine orange staining method. It has been established that histological analysis is very suitable for nanomedicine, which does not cause apparent stress like swelling and inflammation organs of the mouse. The findings could offer solid evidence for nanoparticles' apoptotic cell death mechanism and a novel cancer therapy approach.GRAPHICS.