Proteome-wide identification of lysine succinylation in the proteins of Eucommia ulmoides Oliver leaves revealed its involvement in energy metabolism

摘要

The lysine succinylation, evolutionarily conserved post -translational modification (PTM), played an important regulatory role in physiology of both eukaryotes and prokaryotes. Large succinyl groups conjugated to lysine residues resulted in changes in structure and charge of the proteins and affected the function of these proteins. However, little information was available about the lysine succinylation in the Eucommia ulmoides, an important traditional Chinese herb with multiple active substances. In this study, we performed lysine succinylome analysis to determine the potential regulatory role of lysine succinylation in growth and development of E. ulmoides by LC -MS/ MS combined with immune -purification. A total of 653 lysine succinylation sites distributed in 365 proteins were identified. Gene ontology (GO) analysis showed that lysine-succinylated proteins are related to a wide range of cellular functions, from metabolic processes to stimuli responses. Bioinformatic analysis showed that succinylated proteins were mainly distributed in chloroplast (176 proteins), cytoplasm (99 proteins) and mitochondria (33 proteins) which are mainly involved in energy metabolism, including photosynthesis, Calvin -Benson cycle, glycolysis and TCA cycle. Our research suggested that lysine succinylation was necessary for life activities, which were mainly involved in the process of converting solar energy into biomass energy stored and converting the energy stored by plants into energy (ATP) available for direct use in E. ulmoides. This finding provided a research basis for further revealing the mechanism of lysine succinylation regulating various physiological activities in plants.