Origanum munzurense (O. munzurense) is an endemic species of Tunceli region of Turkey. In this study, we investigated in vitro anticancer effect of aqueous extract of O. munzurense (OME) on breast cancer cells (MCF-7). In vitro cytotoxic effect of OME was evaluated in MCF-7 cells by MTT assay. The wound-healing assay was used to examine the inhibition of migration. Annexin V/propidium iodide staining, cell-cycle distribution was assessed by flow cytometry for MCF-7 cells treated with OME. MTT results show that OME demonstrated in vitro cytotoxicity with 600 mg at 48 h on MCF-7. Doses of 400 mu g/mL and 600 mu g/mL OME significantly suppressed the migration rate of MCF-7 cells. OME significantly decreased the percentage of live cancer cells and showed an apoptotic effect after 48 h of incubation. These results show that OME is effective against breast cancer when administered at high doses and for a long time.
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