Synthesis of zinc oxide nanoparticles using Ficus thonningii aqueous extract and evaluation of its anti-oxidant and anti-microbial activities

摘要

Zinc oxide nanoparticles (ZnO-NPs-AE) were synthesized using Ficus thonningii aqueous extract as an efficient stabilizing agent. Anti-microbial and anti-oxidant activity of the synthesized ZnO-NPs-AE were carried out using disc diffusion and DPPH.(2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical method, respectively. Characterization of the synthesized ZnO-NPs-AE was achieved using UV-vis spectroscopy (UV-vis), Fourier-transform infrared spectroscopy (FTIR). X-ray diffraction (XRD), Scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX). Absorption peak in the range of 370 nm was observed using UV-vis following a color change from yellow to brown; both UV-Vis and FTIR spectra confirmed the formation of ZnO-NPs-AE, and SEM analysis result revealed spherical morphology with an average size of 18 nm. Anti-microbial analysis result of the biosynthesized ZnO-NPs-AE showed significant activity against Escherichia coli and B. cereus with lower concentrations. Moreover, the anti-oxidant activity result obtained using DPPH free radical scavenging method of the ZnO-NPs-AE revealed an IC_(50) value of 35 μg/mL. Biosynthesized ZnO-NPs-AE displayed anti-oxidant activity and anti-microbial potential against both gram-negative and gram-positive bacteria.