We studied the growth-stimulating effect of platelet-based preparations obtained by different methods on cultured human fibroblasts M-22. Platelet lysates prepared from platelet-rich plasma, platelet-poor plasma, concentrated suspension of platelets washed from plasma, and platelet-rich plasma activated with calcium chloride (ActPRP) were used. The volume of the platelet preparations was 10-500 mu l per 10(4) cells. The most effective dose of platelet-rich and platelet-poor plasma in cell culture was 20 mu l, whereas for ActPRP, the most effective dose was 500 mu l. Lysates of platelet-rich and platelet-poor plasma in doses of 100-500 mu l inhibited fibroblast growth and disturbed their structural integrity. At the same time, lysates of washed platelets in doses of 10-500 mu l stimulated cell growth and preserved their viability. An inverse correlation was found between the number of cells in culture and the level of proinflammatory cytokines in the platelet preparations.
展开▼
