CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H

摘要

We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL−1, which is quite superior to those of alternative approaches.