Implanting of Single Zinc Sites into 2D Metal–Organic Framework Nanozymes for Boosted Antibiofilm Therapy

摘要

Design of nanozymes with catalytic active sites at atomic-scale not onlyimproves the atomic utilization, but also provides a well-defined coordinationstructure for nanozymes’ catalytic mechanism research. Herein, a surfactantassistedmethod is reported for preparing 2D metal–organic frameworksbased single zinc sites nanozyme (SZN-MOFs) through assembling preformedZn single-atom coordinated porphyrin precursors into ultrathinMOF nanosheets. The Zn atom loading weight ratio in SZN-MOFs is up to4.6 wt.. The SZN-MOFs exhibit extraordinary peroxidase-like activity, whichcan effectively catalyze H_2O_2 into hydroxyl radicals. The catalytical mechanismis elucidated and the origin of the high peroxidase-like activity of SZNMOFsis rationalized using density functional theory calculations. Finally, itis demonstrated that the SZN-MOFs present great promises for in vitro andin vivo antibiofilm activity under a low concentration of H_2O_2. This study notonly develops a surfactant-assisted method for fabricating MOF-based singlesites nanozyme, but also manifests the applications in the field of antibiofilmtherapy.