Knee osteoarthritis phenotypes based on synovial fluid immune cells correlate with clinical outcome trajectories

摘要

? 2022 Osteoarthritis Research Society InternationalBackground: Knee osteoarthritis (KOA) is a highly heterogeneous disease encompassing a wide range of clinical phenotypes. Phenotypes based on immune cells and protein pattern in synovial fluid (SF) and their relationship to clinical trajectories have not been described. Objective: To assess phenotypes based on immune cells and protein pattern of SF in KOA. Design: SF-derived immune cells were investigated in 119 patients with KOA using flow cytometry. Immune-phenotypes (iPhen) were determined by multivariate patient similarity network analysis and related to clinical trajectory (3–6 months post-sampling) along with protein pattern and macrophage chemokine receptors. Results: Four iPhen were detected based on the distribution of T-lymphocytes, monocyte–macrophage lineage cells and activated CD8+ T-lymphocytes. The ‘activated’ phenotype (n = 17) had high T-lymphocytes but low monocyte–macrophage lineage cells and neutrophils, all highly activated, and showed improved symptoms in 70% patients. The ‘lymphoid progressive’ phenotype (n = 31) had high neutrophils, low lymphocytes and monocyte–macrophage lineage cells, low activation and was associated with lower pain levels. The ‘myeloid progressive’ phenotype (n = 35) had high NK and monocyte–macrophage lineage cells but low T-lymphocytes and activation. The ‘aggressive’ phenotype (n = 36) had high lymphocytes, macrophages, NK cells and neutrophils and high activation, and only 39% of patients improved during follow-up. Low CXCR4 and CCR7 expression on macrophages and high CXCL10 in SF were linked to improved clinical trajectory. Conclusion: We identified four immune-phenotypes that were associated with different clinical trajectories in KOA patients. How these phenotypes can be targeted therapeutically deserves further investigation.