Procedures forSxsAntigen Detection by Antibody-Mediated Cytotoxicity Tests. A Comparative Analysis

摘要

Biological reagents used in the serological detection ofSxsantigen by antibody-mediated cytotoxicity tests were compared in order to optimize the method. Our analyses showed that: (a) red cell-free spleen cells are the best target cells, (b) rabbit serum used as the complement source should be obtained from females, and absorbed with female spleen cells before use, (c) antiserum obtained by immunizing females with repeated injections of syngenic male spleen cells provides the highest anti-Sxsantibody titer, and (d) of the different biological fluids investigated, testis supernatant has highest concentration ofSxsantigen.