AbstractGroups of five male HA (ICR) mice were injected intraperitoneally with 60, 150, 300, or 600 mg/kg body weight of ethyl methanesulfonate (EMS) or with saline vehicle. Each male was mated to two untreated females at 2 and 5 weeks after treatment. The two successive matings utilized sperm derived from post‐ and premeiotic germ cells, respectively. Progeny were evaluated for litter size, body weight, negative geotactic response, swimming patterns, limb use while swimming, water escape time, and open‐field motor coordination activity. Body weight, geotactic response, limb use, and open‐field behavior test results demonstrated that EMS causes heritable behavior mutations in both post‐ and pre‐meiotic germ cells. Among the tests that showed inherited differences between control and treated groups, the computer‐monitored open‐field behavior test was the mo
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机译:摘要以5只雄性HA(ICR)小鼠为一组,腹腔注射60、150、300、600 mg/kg甲磺酸乙酯(EMS)或生理盐水载体。每只雄性在治疗后 2 周和 5 周与两只未经治疗的雌配。两次连续交配分别利用来自减数分裂后和减数分裂前生殖细胞的精子。评估后代的产仔数、体重、负地向反应、游泳模式、游泳时的肢体使用、逃水时间和开阔场运动协调活动。体重、地向反应、肢体使用和旷场行为测试结果表明,EMS 在减数分裂后和减数分裂前生殖细胞中都会引起可遗传的行为突变。在显示对照组和治疗组之间遗传差异的测试中,计算机监控的开场行为测试是 mo
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