Meristems culture for virus irradiation in potato (Solanum tuberosum) cultivars in Palestine

摘要

© 2022, Gaurav Society of Agricultural Research Information CentreAll rights reserved.Potato production is highly affected by viral diseases such as potato virus X (PVX) and potato virus Y (PVY) which can be overcome by establishing a reproducible meristem culture protocol for the production of virus-free potato plantsThis study was conducted during 2017 and 2018 on two potato cultivars “Spunta” and “Alaska Red” in PalestineThe tubers were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) for detection of virus infectionThe infected tubers for both cultivars were incubated, and after sprouting, meristems-tips were isolated and cultured on Murashige and Skoog (MS) mediumAfter four weeks, developed shoots were cultured on MS media supplemented with different rates of three hormonal combinations (0.54 µM NAA; 0.54 µM NAA + 1.0 µM TDZ; 0.54 µM NAA + 2.2 µM BA; 0.54 µM NAA + 2.3 µM Kinetin; 0.54 µM NAA + 1.0 µM TDZ+ 2.2 µM BA + 2.3 µM Kinetin)The results showed that maximum shoot multiplication was observed when NAA at 0.54 µM was combined with 1 µM TDZ (2.85 and 2.55 shoots) in both “Spunta” and “Alaska Red” cultivars, respectivelyHigher shoot length and leaf number were obtained when NAA was combined with either BA (2.2 µM) or Kinetin (2.3 µM) in both cultivarsIn vitro multiplied shoots were rooted and acclimatized successfullyNeither t PVY nor PVX viruses were detected by RT-PCR on the RNA extracts from the tested in vitro grown samples.