Comparison of Surface Persistence of SARS-CoV-2 Alpha and Delta Variants on Stainless Steel at 4 degrees C and 24 degrees C

摘要

Human transmission is believed to occur primarily through direct transfer of infectious droplets or aerosols. However, fomite transmission through contact with contaminated surfaces may also play an important role. Most studies on surface persistence of SARS-CoV-2 have been conducted at temperatures between 20 degrees C and 30 degrees C. There is limited data on the survival of SARS-CoV-2 at low temperatures. In this study, the stability of SARS-CoV-2 Alpha and Delta variants on stainless steel was investigated at two temperatures (4 degrees C and 24 degrees C). The results show that both variants decayed more rapidly at 24 degrees C compared with 4 degrees C. At 24 degrees C, Alpha and Delta variants showed reductions of 0.33 log(10) and 1.02 log(10), respectively, within the first 2.5 h. However, at 4 degrees C, Alpha variant showed a reduction of 0.16 log(10) within the first 2.5 h while no reduction was observed with Delta variant. After remaining in situ for 24 h at 24 degrees C, log(10) reductions of 2.66 (Alpha) and 3.11 (Delta) were observed. No viable Alpha and Delta variant was recovered after 48 h and 72 h, respectively. After 24 h in a refrigerated environment (4 degrees C) log(10) reductions of 1.16 (Alpha) and 0.95 (Delta) were observed. Under these experimental conditions, both viruses survived on stainless steel for at least 1 week. No viable Alpha and Delta variant was recovered after 10 days. These findings support the potential for increased fomite transmission of SARS-CoV-2 during winter months in colder regions worldwide and in some industrial sectors. IMPORTANCE Human transmission is believed to occur primarily through direct transfer of infectious droplets or aerosols. However, fomite transmission through contact with contaminated surfaces may also play an important role. This study provides novel evidence comparing the stability of Alpha and Delta variants on stainless steel surfaces at 4 degrees C and 24 degrees C. At 4 degrees C both variants were found to be still detectable for up to 7 days. At 24 degrees C Delta variant could be recovered over 2 days compared with Alpha variant which could not be recovered after 2 days. This has implications for fomite transmission interventions for people living and working in cold environments.