A Novel Ratiometric Fluorescent Probe for Hypobromous Acid Detection in Living Cells

摘要

A novel ratiometric fluorescent probe HB-2 based on benzothiazole for hypobromous acid (HBrO) was synthesized and studied. After HBrO was added to the CH3CN-HEPES solution of the HB-2, the probe cyclized to form a sulfimine bond, which increased the conjugated degree of the system. The fluorescence emission peak of the reaction system at 447 nm gradually decreased, while the newly generated emission peaks gradually increased at 580 nm. The ratio of the fluorescence intensity at the two wavelengths of 447 nm and 580 nm increased about 234 times. Moreover, it has successfully been used to stain and record microscopy images of 4T1 cells, and the presence of exogenous HBrO could be judged by the change of fluorescence intensity.