Interaction of chloroquine with 2-(hydroxypropyl)-β-cyclodextr in through the supramolecular assembly for cytotoxicity on breast cancer cell lines

摘要

Our goal in the present study is to investigate the supramolecular assembly of chloroquine (CQ), with the cyclic nature of oligosaccharides, such as 2-(hydroxypropyl)-β-cyclodextrin (2HP-β-CD), in an aqueous medium. Using the BH equation, the binding constant (K) and the stoichiometric ratio between the host, 2HP-β-CD, and guest CQ are determined from the spectral changes observed in the absorption and fluorescence spectra. By employing analytical measurements such as FT-IR, SEM, powder XRD, and DSC techniques, inclusion complexes (ICs) are characterized. In addition, DFT analysis using the B3LYP/6-31G method is used to provide the structural proposal for the CQ complex with 2HP-β-CD (CQ:2HP-β-CD-ICs). Likewise, a test with cancer cell lines on the pure CQ and ICs showed no significant difference between the ICs and CQ alone in terms of cytotoxicity performances.