MOLECULAR DIFFERENTIATION OF BRUCELLA ISOLATES BY REPETITIVE SEQUENCE-BASED PCR

摘要

Brucellosis is a worldwide zoonotic disease of major public health, animal welfare and economic significance. Strain differentiation is very much important for the control and eradication of Brucella. The objective of this study was to perform Entero-bacterial Repetitive Intergenic Consensus (ERIC) sequence-based and Repetitive sequence-based polymerase chain reaction (REP-PCR) for molecular differentiation of field isolates of Brucella. Fingerprinting with ERIC and REP-PCR generated distinct amplifIcation bands ranging from 10-11 and 1-9 bands, respectively. Five profiles (El to E5) were observed for ERIC-PCR with profile E1 as most common having 66 isolates. Out of 32 profiles (R1-R32) observed for REP-PCR, R5 with 7 bands was most common and present in 16 isolates. REP-PCR, in the present study could discriminate isolates at genus and species level.