A Genome Resource for Xylella fastidiosa subsp. multiplex Strain P5A2 Causing Phony Peach Disease in the Southeastern United States

摘要

Xylella fastidiosa is a gram-negative bacterial pathogen infecting many economically important crops including alfalfa, almond, citrus, grape, olive, peach, pecan, and plum (Hopkins 1977, 1989; Purcell and Hopkins 1996; Sicard et al. 2018). In the United States, all known X. fastidiosa strains belong to one of two subspecies, X. fastidiosa subsp. fastidiosa or X. fastidiosa subsp. multiplex (Schaad et al. 2004). X. fastidiosa subsp. fastidiosa causes Pierce’s disease (PD) of grapevine, which is thelimiting factor for the expansion of wine grape industry in the southeastern United States, and PD is currently a significant threat to viticulture in California. Another important X. fastidiosa disease in the southeastern United States is phony peach disease (PPD) (Hopkins 1977, 1989; Hutchins et al. 1951; Johnson et al. 2021; Neal 1920), presumably caused by a strain of X. fastidiosa subsp. multiplex based on 16S rRNA gene sequence (Chen et al. 2000). Unlike PD that has attracted extensive research efforts in the past 20 years, largely due to PD outbreaks in California, PPD has received much less research attention, although peach is also an important crop in the United States, with an estimated value of over $624 million in 2021 (USDA-NASS 2022). There are currently >140 X. fastidiosa whole genome sequences deposited in the GenBank bacterial genome database, but none are from X. fastidiosa subsp. multiplex strains associated with PPD.