A New Approach to the Extraction of Prostaglandins 6-Keto-F1#x3B1;, F2#x3B1;, E2, and E1from Gastric Mucosa with Quantitative Analysis by Reverse Phase Liquid Chromatography

摘要

A simple and reliable method is presented for the extraction of Prostaglandins 6-Keto-F1α, F2α, E2and E1from gastric mucosa with quantitation by Reverse Phase Liquid Chromatography. Extraction is accomplished without the need for the harsh chemicals normally associated with tissue extraction. The efficiency of prostaglandin extraction is demonstrated utilizing radiolabeled prostaglandins. Quantitation of these compounds is compared to measurement by radioimmunoasay.