EStradiol and PRogesterone in In vitro fertilization (ESPRIT): a multicenter study evaluating third- versus second-generation estradiol and progesterone immunoassays

摘要

Purpose To assess estradiol (E_2) and progesterone levels during ovarian stimulation determined by third-generation (Gen III) and second-generation (Gen II) Elecsys? immunoassays. Methods E_2 and progesterone concentrations were measured using Elecsys? Gen III and Gen II immunoassays, and progesterone concentrations on the day of ovulation triggering were determined by LC-MS/MS. This was a retrospective, non-interventional study conducted at European tertiary referral infertility clinics in women aged 18-45 years, with a body mass index 18-35 kg/m~2, regular menses, and both ovaries. Results Serum samples were obtained from 230 women classified by oocyte retrieval as poor (33.0; 0-3 oocytes), normal (40.9; 4-15 oocytes), or high (26.1; >15 oocytes) responders. E_2 and progesterone levels increased during ovarian stimulation, with greatest increases observed in high responders. Elecsys? Gen III and Gen II assay results were highly correlated for E_2 (Pearson's r = 0.99) and progesterone (r = 0.89); Gen III results were lower than Gen II for both E_2 and progesterone. On the day of triggering, Gen III E_2 and progesterone levels showed a difference of- 15.0 and -27.9, respectively. Progesterone levels (on day of triggering) measured by LC-MS/MS correlated better with Gen III (0.98) than Gen II (0.90). Mean relative differences for Gen III and Gen II assays versus LC-MS/MS were 14.6 and 62.8, respectively. υonclusion E_2 and progesterone levels determined with Elecsys? Gen II and III assays were highly correlated; results were lower for Gen III versus Gen II. Differences observed for progesterone on the day of triggering may be clinically relevant.