Establishment of cell suspension cultures of Azadirachta indica for the production of nimbolide

摘要

© 2022, Indian journals. All rights reserved.The present study aimed to establish an effective cell suspension medium for the production of nimbolide from the callus culture derived from leaf explants of Azadirachta indica. The callus was induced in MS basal medium supplemented with different concentrations of auxins and cytokinins and the highest callusing percentage (93.3) was obtained in MS basal medium supplemented with 1.0 mgL–1 IBA and 0.5 mgL–1 BAP. The friable calli were transferred to suspension cultures that were prepared with full strength and half strength liquid MS medium with varying concentrations of IBA and BAP. HPLC analysis revealed the presence of nimbolide in all the cell suspension culture and higher content of about 0.04 was measured in half MS medium supplemented with 1.0 mgL-1 IBA and 0.5 mgL-1 BAP. Thus, this study has identified optimum culture conditions that favours higher amount of nimbolide which has several applications both in agriculture and healthcare industry.