Evaluation of in vitro antioxidant potential in different parts of Syzygium caryophyllatum (L.) Alston

摘要

© 2022, Indian journals. All rights reserved.Studies on oxidative stress and its consequences on human health have gained a lot of attention in recent years. Many diseases are caused by an imbalance between pro-oxidant production and neutralization. Antioxidant principles obtained from plant resources have a wide range of impacts and provide a lot of potential for rectifying the imbalance. These natural antioxidants are recognized to aid in the treatment of variety of human illnesses by inhibiting the formation of free radicals. In the present studies, in vitro antioxidant studies were conducted using methanolic extract of plant parts of Syzygium caryophyllatum (L.) Alston (Myrtaceae). The total polyphenols, flavonoids and tannins were determined after the extraction. DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), NO (nitic oxide), FRAP (ferric reducing antioxidant power) and reducing power assays were done to evaluate antioxidant activities. Maximum levels of polyphenols (431.85±2.34 mg of GAE/g of extract), total flavonoids (201.62 ±3.39 mg of QE/g of extract) and tannins (355.32±1.77 mg of TAE/g of extract) in the bark was recorded. The bark extract showed more scavenging activity with IC50 value for DPPH, ABTS and nitric oxide were found to be 6.20±0.001 μg/ml, 13.785±0.196 μg/ml and 79.855±0.003 μg/ml, respectively. When compared to the standard ascorbic acid that is commonly used to measure antioxidant capacity, the leaf, fruit, seed and bark extracts showed remarkable antioxidant activity. The result of this study are encouraging, suggesting that the bark of S. caryophyllatum can be exploited as a potent antioxidant in a variety of formulations for the treatment of disorders resulting from excessive biological oxidative stress.